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Product Data |
| | mAb to O6-ethyl-2-deoxyguanosine (ER 17) |
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Product Name |
mAb to O6-ethyl-2-deoxyguanosine (ER 17) |
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Synonyms |
ER 17 monoclonal antibody |
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Catalog No |
SQM002.1 |
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Size |
50 µg |
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Price |
375,00 EUR |
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Category |
Antibodies and Conjugates |
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Description |
monoclonal antibody, specific for O6-ethyl-2-deoxyguanosine |
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Background |
DNA-lesion specific monoclonal antibody |
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Feature |
Detects a specific mutagenic DNA modification induced by several exogenous and endogenous carcinogens e.g. food, smoking, cancer therapeutics, environmental carcinogens, workplace carcinogens; Molecular epidemiology of carcinogen exposure; Pre- and intratherapeutic dosimetry of exposure to anticancer agents; Basic research of molecular mechanisms of carcinogenesis; Mutagenicity testing of substances |
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Molecular Weight |
800 kDa |
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Presentation |
lyophilized |
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Reconstitution |
reconstitute with 0.5 ml of PBS, 0.05% sodium azide, 1% bovine serum albumine |
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Stabilizer |
none |
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Storage |
-20°C |
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Applications |
competitive radioimmunoassay; Immunocytochemistry: 0.05 - 0.2 µg/ml in PBS containing 3% BSA; ELISA: 0.2-0.5 µg/ml PBS containing 3% BSA |
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Characteristics |
specifically reacts with O6-ethyl-2-deoxyguanosine in human, mouse, rat |
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Host |
rat |
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Isotype |
IgM |
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Clonality |
monoclonal |
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Clone |
ER 17 |
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Immunogen |
O6-ethyl-2-deoxyguanosine-Thyroglubulin conjugate |
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Specificity |
O6-ethyl-2-deoxyguanosine |
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Cross Reactions |
see RIA specifity table (see graphics) |
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Affinity Constant |
2.2 x 10 e10 (l/Mol) |
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Purification |
Protein G affinity purification from cell culture supernatant |
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Purity |
>95% |
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Working Dilution |
test dependent |
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Expiry |
1 year |
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Considerations for Use |
Please store the vial at 4-8°C. The product is stable for 1 year at 4-8°C in its stock solution. Working dilutions should always be prepared freshly. For extended storage of the stock solution please add 50% glycerol and freeze the product. |
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Keywords |
mutagenic DNA-modification; mAb, monoclonal antibody to modified DNA-base; mutagenicity testing, cacinogenesis, intratherapeutic dosimetry, molecular epidemiology; DNA-adduct; O-alkyl adduct detection in DNA |
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| Squarix GmbH - Elbestraße 10 - D-45768 Marl - Germany |
| fon: +49-2365-20400-0 - fax: +49-2365-20400-60 - www.squarix.de - e-mail: info@squarix.de |